adipocyte derived hormones gene expression, resistin and visfatin, in ags gastric cancer cell line

background: adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines.  it has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer.  this study has managed to investigate endogenous and/or exogenous expression of visfatin and resistin in gastric cancer cell line. methods: cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mrna and protein expression of resistin and visfatin in gastric cancer cell lines. elisa test has performed for cell lysate and supernatant of cell culture to measure resistin and visfatin protein expression and secretion. results: human gastric cancer cell line (ags cell line) has found to express visfatin mrna and protein but resistin mrna and protein has not expressed. conclusion: visfatin has expressed endogenously in ags human gastric cancer cells. conversely resistin has no expression. the results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer. please cite this article as: gorgian mohammadi m, hedayati m, zarghami n, ghaemmaghami s, mohaddes m. adipocyte derived hormones gene expression, resistin and visfatin, in ags gastric cancer cell line. iran j cancer prev. 2013; 6(3):165-9. references 1. crookes pf. gastric cancer . clin obstet gynecol. 2002; 45: 892-903. 2. bianchini f, kaaks r vainio h. overweight, obesity, and cancer risk . lancet oncol. 2002; 3: 565-74. 3. calle ee, kaaks r. overweight, obesity and cancer: epidemiological evidence and proposed mechanisms . nat rev cancer. 2004; 4: 579-91. 4. gunter mj, leitzmann mf. obesity and colorectal cancer: epidemiology, mechanisms and candidate genes . j nutr biochem. 2006; 17: 145-56. 5. trayhurn p, beattie jh. physiological role of adipose tissue: white adipose tissue as an endocrine and secretory organ . proc nutr soc. 2001; 60: 329-39. 6. wozniak se, gee ll, wachtel ms, frezza ee. adipose tissue: the new endocrine organ? 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